Multiomic Analysis Provided Insights into the Responses of Carbon Sources by Wood-Rotting Fungi Daldinia carpinicola

Multiomic Analysis Provided Insights into the Responses of Carbon Sources by Wood-Rotting Fungi Daldinia carpinicola

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SMEG C92DX9 90 cm Dual Fuel Range Cooker - Stainless Steel LPG Convertible Daldinia carpinicola is a newly identified species of wood-rotting fungi, with substantial aspects of its biology and ecological function yet to be clarified.A Nanopore third-generation sequencer was employed for de novo genome assembly to examine the genetic characteristics.The genome consisted of 35.

93 Mb in 46 contigs with a scaffold N50 of 4.384 Mb.Glycoside hydrolases and activities enzymes accounted for a large proportion of the 522 identified carbohydrate-active enzymes (CAZymes), suggesting a strong wood degradation ability.

Phylogenetic and comparative analysis revealed a close evolutionary relationship between D.carpinicola and D.bambusicola.

D.carpinicola and Hypoxylon fragiforme exhibited significant collinear inter-species genome alignment.Based on transcriptome and metabolomic analyses, D.

carpinicola showed a greater ability to utilize sucrose over sawdust as a carbon source, enhancing its growth by activating glycolysis/gluconeogenesis and Monitor Wire Harness the citrate cycle.However, compared with sucrose, sawdust as a carbon source activated D.carpinicola amino acid biosynthesis and the production of various secondary metabolites, including diterpenoid, indole alkaloid, folate, porphyrin, and biotin metabolism.

The study establishes a theoretical basis for research and applications in biological processes, demonstrating a strategy to modulate the production of secondary metabolites by altering its carbon sources in D.carpinicola.